Coding
sinI

Part:BBa_K594001

Designed by: Yu Xiaoyan   Group: iGEM11_OUC-China   (2011-09-30)


a luxI homologous AHL synthetase from Sinorhizobium meliloti 1021

The Sinorhizobium meliloti strain Rm1021 harbors at least two quorum-sensing systems; one of them is sinR/sinI system, The sinI is responsible for the production of several novel AHLs, including C12-HSL, 3-Oxo-C14-HSL, C16:1-HSL, 3-Oxo-C16:1-HSL, C18-HSL, some of theses are the longest AHLs identified so far. The sin system correlates with a delay in the appearance of nitrogen-fixing nodules, as well as with an overall decrease in the number of pink nodules, suggesting a role for quorum sensing in establishing a successful symbiosis with Medicago sativa.

As a autoinducer synthatase, the use of sinI is the same with luxI, namely to produce AHLS.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//classic/signalling/sender
Parameters
chassisDH5α